Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (2): 215-220.doi: 10.3969/j.issn.2095-4344.2017.02.010

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Preparation of polyvinyl alcohol/lota-carrageenan scaffolds and its biocompatibility

Cui Jing1, Zhang Ya-bin2, Ma Si-qi1, Xiong Yan-jie3, Cui Man1, Li Shuo-feng1, Che Peng-cheng1, Yao Fang-lian2,Sun Hong1
  

  1. 1Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 3the Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province China
  • Received:2016-10-27 Online:2017-01-18 Published:2017-02-27
  • Contact: Sun Hong, Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Cui Jing, Studying for master’s degree, Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81101448, 51573127; the Natural Science Foundation of Hebei Province, No. C2011401006, H2012401017; the Project of Overseas Students Introduction in Hebei Province, No. C201400560

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Abstract:

BACKGROUND: Polyvinyl alcohol (PVA) hydrogel with similar porous structure and mechanical properties to the natural cartilage is very suitable for the repair of articular cartilage. However, the pure PVA hydrogel after lyophilization will be accompanied by the shrinkage of the polymer network and the collapse of the pores, leading to the inhomogeneous performance of the material even in the state of re-swelling. Addition of the active polymer will increase the cell adhesion ability of PVA hydrogel.
OBJECTIVE: To construct PVA/ lota-carrageenan (l-CA) composite materials with different mass fractions of l-CA and evaluate the biocompatibility with vascular endothelial cells.
METHODS: PVA/l-CA composite films with different contents of l-CA were fabricated and then co-cultured with vascular endothelial cells. Attachment, proliferation and morphological changes of vascular endothelial cells on the composite were observed by scanning electron microscope and MTT assay to evaluate its biocompatibility. PVA/l-CA three-dimensional scaffold with different contents of l-CA were constructed, and hemolysis experiment was conducted according to the biological evaluation standards of medical devices, and the porosity and pore size were observed using scanning electron microscope.
RESULTS AND CONCLUSION: In vitro experimental results showed that the addition of l-CA could significantly increase the biological activity of PVA hydrogel, and promote the cell attachment and proliferation on the scaffold. The hemolysis rate of each experimental group was less than 5% (the accepted safety standard), suggesting that the composite materials were in accordance with the standard of medical devices for hemolysis experiment. These findings indicate that the composite scaffolds with 20%-30% l-CA possess the pore size suitable for cell growth and proliferation and the porosity beneficial for transportation of nutrients and metabolites, which can serve as an excellent scaffold for tissue engineering.

Key words: Endothelial Cells, Hydrogel, Biocompatible Materials, Tissue Engineering

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